Equipment
Real-time PCR (ABI QuantStudio7 Pro)
The apparatus enables accurate detection and quantification of nucleic acids using fluorescent dyes and enables the analysis of 384 samples at the same time, as well as the analysis of 96 samples in reaction volumes of up to 100 ul when the thermocycler blocks are changed. The apparatus excites dyes in the wavelength range of 450-600 nm and detects dyes in the range of 500-640 nm, which enables the simultaneous detection of at least five dyes with the necessary algorithm to accurately distinguish between several components of multi-color signals. In this way, it enables accurate simultaneous detection of at least five different DNA sequences (multiplex quantitative reactions). The apparatus detects one copy of the target molecule and provides linearity over 10 logarithmic orders.
Responsible for equipment:
Katja Stare
URL:
http://www.nib.si/infrastruktura/infrastrukturni-center-planta
The equipment is a part of Infrastructural program NIB. For the access of the equipment contact the Head of IC Planta (http://www.nib.si/eng/index.php/infrastructure/infrastructural-centre-planta). Waiting time for the access of the equipment is up to 1 month.
Research projects (1)
Legend
Organisations (1)
| no. |
Code |
Research organisation |
City |
Registration number |
No. of publicationsNo. of publications |
| 1. |
0105 |
National Institute of Biology |
Ljubljana |
5055784 |
0 |
