The cells and tissues respond to physical stimuli such as electromagnetic fields. The response depends on the parameters of electromagnetic field, on the cell and tissue characteristics, and other factors such as temperature, the density of the cells and their interactions. In the frame of our research programme we investigated the use of electric currents for tumour healing and acceleration of chronic wound healing. The cell in electric field moves, orients, rotates, and when the electric field intensity is high enough electroporation takes place. Electroporation is a phenomenon which consequence is increased permeability of cell membrane or fusion of cells which are in contact. Different levels of biological complexity from lipid bilayers, cell membrane, cells and tissues to entire organism were studied and the results were used in biomedical purposes. The most important results are as follows: 1.) electroporation and electrochemotherapy: Development of a new method for detection of permeabilized cells and study of the effect of different pulses, electroporation medium and molecules that change membrane properties on the efficiency of cell membrane permeabilization for exogene molecules. In collaboration with the Institute of Oncology in Ljubljana we performed studies for introduction of cisplatinium into electrochemotherapy. 2.) the effect of electrotherapy on tumour oxygenation: We focused on the introduction of non-invasive optical methods for the measurements of oxygenation in the tissue. We introduced the use of the laser Doppler method for blood perfusion measurements on the level of microcirculation and luminescence oxymetry for measurements of partial pressure of oxygen in the tissue. The assumption that the reduction of blood flow in tumours caused by electroporation pulses besides direct effects on tumour cells has indirect effects caused by the reduction of blood flow was confirmed. 3.) interstitial fluid pressure (IFP) in tumours: The main finding was that some vasoactive substances affect IFP and their effect is inversely correlated with the tumour size. 4.) electric stimulation of wound healing: On the basis of acquired knowledge about the dynamics of wound healing, patient and wound characteristics we developed the model that predicts the time to complete wound closure. 5.) analytical and numerical modelling: Mathematical models contributed to better understanding of electroporation, electrochemotherapy, IFP and wound healing predictions and they were used for electrode design evaluation and for validation and development of different procedures for electrochemotherapy. 6.) the hardware and software development: Prototypes of electroporators, module for gene transfection, high-speed high-voltage amplifier, that in combination with function generator for generation of high-voltage pulses of desired shapes, system for electroporation of lipid bilayers and the programme for modelling of dynamics of electriporation were developed. The results of the research programme were published in numerous international journals and scientific meetings and published works were cited many times. The members of the programme group were invited lecturers, book chapter's writers, organizers of international meetings and received international and national rewards. The group contributed to software and hardware development of clinical electroporator in European project Cliniporator and collaborated in other European projects and proposals within 6th framework.