Conformational changes of proteins, involved in disease: - we have discovered that stefin A forms symmetrical dimers through domain swapping mechanism and assignated and in collaboration determined its structure - determination and comparison of molecular dynamics of monomeric and dimeric stefin A as the first example of comparison of domain-swapped and monomeric protein, discovery of long-range effects on dynamics - discovery and characterization of complex formation between enzyme precursor and protein inhibitor Research of LPS receptors in the system of innate immunity - expression and refolding of functional recombinant fragment of CD14 in bacterial cells, preparation of CD14 in P.pastoris - identification of peptide fragment of MD-2, which binds LPS, its antimicrobial activity - identification of MARCKS as intracellular LPS receptor, involvement of LPS in actin depolymerization, determination of LPS binding site on MARCKS effector domain - expression and refolding of recombinant MD-2 in E.coli, preparation of mutants based on 3D model Antimicrobial compounds - use of gyrase B fragment for design of novel antimicrobial compounds as gyrase inhibitors (use of NMR, fluorescence, enzymatic activity), discovery of flavonoids as inhibitors of ATPase activity - identification of flavonoid binding site on gyrase B - determination of tertiary structure of polymyxin B and E as the prototype peptide antibiotics bound to LPS - establishment of the molecular model for LPS neutralization - preparation of recombinant and isotopicaly enriched antimicrobial peptides in bacteria in form of insoluble fusion protein - enhancement of antimicrobial and LPS-neutralizing activity of peptides based on lactoferrin (LF11), determination of optimal length of alkyl chain - determination of tertiary structure of LF11 in complex with LPS and in SDS and DPC micelles - determination of tertiary structure of C12 acylated LF11 in DPC micelles Use of NMR in biological systems - assignment and 3D structure of cytochrome c of P.denitrificans in reduced and oxidized state - determination of interactions between cytochrome c fragments - determination of the mechanism of electron transport in the system of mitochondrial steroid dehydrogenase - determination of 3D structure of RcsB and characterization of ins interaction with DNA - program for automatic assignment of NMR spectra based on the structural model - implementation of SNIF-NMR method for wine analysis in combination with IRMS, identification of aminoacids in wine samples - determination of minor constituents in wine using NMR, extension to the analysis of fruit juice and olive oil