A continuous flow through system was presented as a lab exercise at the 2017 EBTT international postgraduate course. E.coli bacteria was inactivated in a continuous flow through system using different parameters of electric pulses. Thus the current results of the project group were presented directly to the next generation of researchers in the area of electroporation.
F.18 Transfer of new know-how to direct users (seminars, fora, conferences)
COBISS.SI-ID: 11902292Extraction of proteins and plasmid DNA by means of electroporation was presented at the international symposium on bioelectrochemistry and bioenergetics in Malmo, Sweden. The optimization of the protocol was also suggested. The results of the project were presented to a wider research community.
F.18 Transfer of new know-how to direct users (seminars, fora, conferences)
COBISS.SI-ID: 11114580We have found that for certain parameters of electric pulses plasmid DNA can be extracted from bacterial cells. Therefore we filed a patent application together with company Biaseparations.
F.32 International patent
COBISS.SI-ID: 1561485Within the doctoral thesis, the candidate developed a prototype electroporator, which enables continuous pulse treatment with high voltages (up to 5000 V) and high current values (up to 100 A).
D.09 Tutoring for postgraduate students
COBISS.SI-ID: 11883860Student learned new technique (electroextraction) and studied the influence of different electric pulse parameters on extraction of proteins from E. coli. He presented his work in graduate thesis.
D.10 Educational activities
COBISS.SI-ID: 10655060