Magnetic NPs are a special type of nanoparticles (NPs) with a ferromagnetic, electron dense core that enables several applications like cell tracking, hyperthermia and magnetic separation, etc and as well as multimodality (drug delivery, imaging). Besides NP properties, the NP-cell interactions are also highly dependent on the physiology of the specific cell type. In this study, we analyzed the cellular responses to magnetic cobalt ferrite NPs, coated with polyacrylic acid (PAA) in three cell types: Chinese Hamster Ovary (CHO), mouse melanoma (B16) cell line, and primary human myoblasts (MYO). We compared the internalization pathway, intracellular trafficking and intracellular fate of our NPs using fluorescence and transmission electron microscopy (TEM) as well as quantified NP uptake and analyzed uptake dynamics. We determined cell viability after 24 h or 96 h exposure to increasing concentration of NPs. Our NPs have been shown to readily enter and accumulate in cells in high quantity using the same two endocytic pathways; mostly by macropinocytosis and partially by clathrin-mediated endocytosis. The cell types differed in the uptake rate, the dynamics of intracellular trafficking and the uptake capacity as well as in their response to higher concentrations of internalized NPs. The observed differences in cell responses stress the importance of evaluation of NP-cell interactions on several different cell types for better prediction of possible toxic effects in different cell and tissue types in vivo.
COBISS.SI-ID: 10646612