Solid lipid nanoparticles of various compositions were produced by melt-emulsification method using a rotor-stator homogenizer. A drug with known antioxidative potential, namely resveratrol, was incorporated in the nanoparticle formulation, which demonstrated the most promising physical properties. In order to achieve the prolonged drug release solid lipid nanoparticles were incorporated by electrospinning in polyvinyl acohol nanofibers. Nanofibers containing nanoparticles with resveratrol (composite nanofibers) and nanofibres with incorporated pure resveratrol (simple delivery system) were produced for comparison. Nanofiber morphology, efficiency of drug incorporation and release of the resveratrol were compared. By optimizing the parametres of electrospining homogeneous nanofibers were produced. The nanofiber homogeneity is influenced by both electrospining parameters and the amount of incorporated resveratrol in the fibers. When 0.94 % (w/w) of resveratrol was incorporated in nanofibers in the form of solid lipid nanoparticles, it resulted in the formation of bead, while in the case of nanofibers with resveratrol it resulted in the crystallisation of the drug crystals on the surface of the nanofibers. It was confirmed that nanofibers enable prolonged drug release for several days. The release has been further prolonged by incorporation of resveratrol loaded solid lipid nanoparticles in nanofibers.
D.10 Educational activities
COBISS.SI-ID: 3663729A method for preparing nanoparticles is provided, which comprises the steps ofdissolving a polymer and, optionally, at least one additional ingredient, inan organic solvent, passing the solution through a vibrating nozzle and dropping the solution through the electrical field into an aqueous solution, which is stirred, such that nanoparticles are formed by the rapid diffusion ofthe solvent.
F.32 International patent
COBISS.SI-ID: 3689585Nanofibers have been developed, prepared with the electrospinning method from biocompatible and biodegradable polymers, which would be due to their morphological resemblance to natural extracellular matrix capable of triggering specific cell responses and are therefore an attractive form of material for development of tissue substitutes and drug delivery systems. The most commonly used method for nanofiber production is electrospinning, which is difficult to manage due to numerous interrelated parameters influencing the process. Therefore, the development of electrospun nanofibers with suitable properties is time-consuming and expensive. The electrospinning process has been optimized and emphasis was placed on the effects of solution, process and ambient parameters. Electrospun nanofibers were also morphologically and mechanically characterized, and their potential applicability in tissue engineering was evaluated. We demonstrated a significant correlation between the properties of nanofibres and the response of cells during growth on them.
D.09 Tutoring for postgraduate students
COBISS.SI-ID: 3619441