We reported the design of the genetic regulatory network that detects proteolysis over digit -specific cleavage of the HIV-1 protease , which activates gene transcription , which may be linked to the expression of the gene of the selected effector . This method of construction of the logic circuit is designed on the basis of the identification of the essential functions of the virus , and is independent of the mutation. This principle could be used for the detection of other viral or microbial proteases as well as other essential functions and represents a good example of the application of the principle of synthetic biology .
COBISS.SI-ID: 5607450
We have described a new principle of regulation of gene transcription via cyclization TALE domains being topologically locks to the DNA or by cyclization prevent them from binding to DNA. We have shown that the absolute level of activation is significantly higher over the two-hybrid principle and reached more than 300 - fold increase in gene transcription.
COBISS.SI-ID: 5839898