The first droplet-digital-PCR-based absolute quantification of “flavescence dorée” phytoplasma, agent of a quarantine phytoplasma yellows in grapevine was performed. It was shown that the assay could be used for quantification and quality control of DNA based on in-house reference materials typically used in diagnostics and metrological laboratories. This new tool has great potential for monitoring phytoplasma kinetics, such as there relationship with the progress of an infection, and variations of the phytoplasma titer through the season and screening plants for resistance.
COBISS.SI-ID: 3174991
Potato virus X is one of the most widespread viruses of potato. However, little is known about its diversity in its likely center of radiation, the Andean region of South America. To fill this gap, the strategy of Illumina deep sequencing of small RNAs was used to obtain complete or near complete genome sequence of PVX from symptomatically infected plant samples from Peru. A complete genome sequence of a representative of a new PVX phylogenetic lineage was obtained, which shows a high level of sequence dissimilarity to other completely sequenced isolates. One of the field samples was infected with the mixture of two PVX strains, which were efficiently discriminated using small RNA sequencing approach. The study confirms the utility of small RNAs deep sequencing for successful viral strain differentiation and discovery of new viral strains and indicates a high diversity of PVX in the Andean region of South America, a pattern which may be expected also for other potato pathogens.
COBISS.SI-ID: 3188559
Severe chlorotic mottling, yellow chlorotic spots and patches were observed on a red raspberry plantation near Smolyan (south Bulgaria). Raspberry leaf blotch virus (RLBV) was detected in the samples. To our knowledge, this is the first report of RLBV in red raspberry in Bulgaria.
COBISS.SI-ID: 4516456