We have presented our results in the field of plant virus population changes tracking by using small RNA sequencing in the frame of the conference of the American Society of Microbiology on the topic of next generation sequencing for molecular epidemiology studies of pathogens.
F.10 Improvements to an existing technological process or technology
COBISS.SI-ID: 3684431RNA viruses have small, simple genomes, which have a high level of diversity and present a special challenge for whole genome sequencing. The problems with virus sequencing can be avoided by utilizing next generation sequencing; a high-throughput sequencing methodology which generates millions of sequences simultaneously from one sample. Due to the low percentage of viral RNA in relation to host RNA in most samples, obtaining the quantity of viral RNA required by routine protocols can be problematic. Despite the depletion of ribosomal RNA from total RNA extracted from raspberry leaves, data analysis revealed a high percentage of plant sequences remaining in the sample. Using size selection during library preparation significantly improved viral to plant RNA ratio. As a result, better coverage of viral genome was achieved with the same amount of total generated data.
F.10 Improvements to an existing technological process or technology
COBISS.SI-ID: 3552591We have presented possibilities for quick detection of plant pests using LAMP mathod in the fram of the "Health Checks and Smart Treatments for our Plants" event.
F.18 Transfer of new know-how to direct users (seminars, fora, conferences)