On the basis of sequence data, pairs of primers were designed, which allow us to discriminate between different isolates of RBDV, cloned in the plasmids. Analyses showed that at least two primer pairs would be needed for specific discrimination between different genotypes of virus.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 31036889Sequencing of small RNAs was shown to be an efficient approach for detection of plant viruses, using NGS. We used the method for characteristaion of new strain of Potato virus X (PVX) in plant samples in Peru. We also establish a lab protocol for isolation of small RNAs from plant samples and succesfully isolated small RNAs from the plants, which were also used for purification of viral particles.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 2824527