The invention provides a modified DNA sequence for the in vitro expression of a recombinant protease from Aeropyrum pernix K1, pernisine, in a heterologous expression system. Furthermore, the present invention relates to a method of preparing the subtilisin-like protease by use of the recombinant DNA techniques.
F.33 Slovenian patent
COBISS.SI-ID: 4484984In her PhD thesis, Eva Ogorevc PhD., studied transfer of microvesicles (MV) between the cells as one form of intercellular communication. The results of her research showed, that MVs in the peripheral blood could be used as non-invasive diagnostic biomarkers for following the disease progress and treatment effectiveness in patients with cancer. Concentration of MVs in patients diagnosed with gastric cancer is namely increased when compared to healthy controls or patients with other types of gastrointestinal diseases. By showing that MVs can transfer from highly malignant to the non-malignant urinary cell line, we have proposed how transformation into cancer phenotype could be induced even in the absence of genetic changes in the cell. The above results contribute to our understanding of mechanisms of microvesiculation and of intercellular communication through MVs.
E.01 National awards
COBISS.SI-ID: 10378580Despite the use of effective highly active antiretroviral therapy, there is a high incidence of neurological symptoms in HIV-infected patients, occurrence of which is still unresolved. At the meeting, attended by all European research groups working on HIV-viral protein Nef, we have presented our current results on the topic of Nef and extracellular vesicles (exosomes). We showed that Nef-expressing or HIV-infected human microglia cells release exosomes containing protein Nef (Nef-exosomes), whereas this is only observed for HIV-infected human astrocytes. Nef-exosomes target cells of the central nervous system (CNS) and cause damage to differentiated CNS cells and also impair the ability of the brain to repair the damage by disturbing the differentiation of neural stem cells. This finding opens new perspectives in understanding of HIV neuropathogenesis.
B.04 Guest lecture
COBISS.SI-ID: 31586009