By means of the LysM-meidated heterologous expression of darpins on the surface of LAB, a fusion protein, bioproduced with recombinant L. lacits, as well as by combining non-rcombinant Lb. salivarirus with the purified expressed fusion protein, a bypass system of non-recombinant heterologous LABs was created.
COBISS.SI-ID: 28301607
In vivo imaging of orally administered lactic acid bacteria (LAB) and commensal bacteria in mice is shown to provide information on the spatial and temporal distribution of bacteria in the gastrointestinal tract. The bacteria can be detected and monitored using bioluminescence or near-infrared fluorescence. The present work provides a methodological basis for future studies of probiotic and theranostic actions of LAB in mouse disease models.
COBISS.SI-ID: 3978609
Non-immunoglobulin (non-Ig) scaffolds are, in contrast to antibodies, small single-domain proteins that require no post-translational modification, often lack disulfide bonds, and can undergo straightforward multimerization. Among the 20 different types of non-Ig scaffolds, Adhirons, Alphabodies, Centyrins, Pronectins, Repebodies, Affimers, and Obodies have been introduced in the past4 years. 102 proteins have been specifically targeted by 139 different non-Ig scaffold binders. The most frequent application of non-Ig scaffolds is in the treatment and diagnosis of cancer and inflammatory diseases, and 10 non-Ig scaffolds have already been tested in clinical trials. Recently, non-Igscaffolds have often been used in research as structure determination chaperones, for intracellular monitoring of post-translational modifications, and as antibody alternatives for microscopy, flow cytometry, and Western blotting.
COBISS.SI-ID: 3851377