During abscission in tomato different processes occur in distal part of leaf or flower petiole that will fall off, compared to proximal part that remains on the plant. An important process in distal part is programmed cell death, where nucleases and enzymes of oxidative stress are expressed. Cells in the proximal part are alive and metabolically active. In abscission zone we observed expression of enzymes that degrade middle lamella and rapid changes in expression of enzymes involved in ethylene metabolism.
COBISS.SI-ID: 28604199
We describe the impact of endoreduplication level on adventitious plant regeneration from explants of pumpkin cotyledons. Endoreduplication is a variant of cell cycle where DNA is duplicated several times. Endoreduplicated cells no longer divide, therefore the regeneration ability is negatively affected.
COBISS.SI-ID: 7983737
In Europe, phytoplasma cause major damage on grapevine, therefore we developed a fast and efficient method for detection using isothermal nucleic acid amplification (LAMP) that can be performed in the field. LAMP assay was shown to the specific and sensitive, comparable to quantitative real-time PCR detection.
COBISS.SI-ID: 4088399
The first one-step reverse-transcription droplet digital PCR-based absolute quantification of RNA viruses in different types of surface water samples is reported in this paper. This quantification method proved to be more precise and more tolerant to inhibitory substances than the benchmarking reverse-transcription real-time PCR (RT-qPCR). This new tool is fully amenable for the quantification of RNA viruses in the particularly low concentrations usually found in water samples.
COBISS.SI-ID: 2990415
The method described is a rapid, total DNA extraction procedure applicable to a large number of plant samples. The procedure combines a simple and quick homogenization step of crude extracts with DNA extraction based upon the binding of DNA to magnetic beads. DNA is purified in an automated process in which the magnetic beads are transferred through a series of washing buffers. The eluted DNA is suitable for efficient amplification in PCR reactions.
COBISS.SI-ID: 2614095