The paper gives an overview on the virus particle production challenges and chromatography based analytical methods for efficient monitoring of the virus production, covering also methods developed in the programme.
COBISS.SI-ID: 4534648
In the paper we report a successful use of sample displacement chromatography (SDC) to separate plasmid DNA (pDNA) isoforms under overloading conditions, where supercoiled isoform acts as a displacer of open circular or linear isoform. This study identifies SDC as a promising new approach to large-scale pDNA purification, which is compatible with continuous, multicolumn chromatography systems, and could therefore be used to increase productivity of pDNA production in the future.
COBISS.SI-ID: 4025851
The goal of the present work was a development of a hydrophobic methacrylate monolith with improved resolution for pDNA isoforms separation and removal of host contaminants. After a detailed search for an appropriate ligand, a pyridine-modified monolithic support was chosen and tested under the conditions of HIC chromatography. Pyridine and C4 HLD monoliths were equally efficient for the removal of the main process impurities, but pyridine exhibited higher purity in terms of sc pDNA homogeneity, showing to be a suitable alternative to C4 HLD for this polishing step.
COBISS.SI-ID: 3996923
In this work quaternary amine methacrylate monoliths were exploited to remove enteric viruses from wastewater treatment plant effluent. Wastewater effluent samples were successfully purified from five waterborne enteric viruses (rotavirus, norovirus genogroup I and II, astrovirus, sapovirus) below the detection limit of RT-qPCR. The obtained results confirm that methacrylate monoliths can be a valuable tool for simultaneous removal of different waterborne viruses from contaminated water sources.
COBISS.SI-ID: 3299151