The lecture systematically presented chromatography of biopharmaceuticals. We compared the challenges and limitations of the chromatography of small and large biological molecules (proteins, viruses, DNA). The lecture is part of a wider programme, where the importance and usefulness of biochromatography is advertised.
B.04 Guest lecture
COBISS.SI-ID: 4072699In invited lecture we presented to international community concepts and importance of large biomolecules analytics in production processes as well as technology development trends, covered also by this programme. The lecture is part of a wider programme, where the importance and usefulness of process analytical technology in biopharmaceutical industry is presented.
B.04 Guest lecture
COBISS.SI-ID: 4029435In invited lecture we presented to international community concepts and importance of immunoaffinity chromatography on chromatographic monoliths, which development is covered also by this research programme. The lecture is part of a wider programme, where the importance and usefulness of immunoaffinity chromatography in biopharmaceutical industry is presented.
F.10 Improvements to an existing technological process or technology
COBISS.SI-ID: 4049659Various techniques have been applied for human serum albumin depletion, such as ultrafiltration, size-exclusion chromatography and affinity-based removal by immobilized antibodies. Despite their high specificity, affinity methods usually suffer from long processing times due to low flow rates supported by chromatographic resins. Here we describe an affinity-based method for improving sensitivity of biomarker detection by using an albumin-specific antibody immobilized onto CIM monolith support. Due to their unique chromatographic properties, the use of monolithic columns enables very fast depletion (approx. 2 minutes per depletion cycle) and column reconditioning, thus significantly increasing sample preparation throughput.
F.06 Development of a new product
COBISS.SI-ID: 4157691At the conference, we presented results clearly showing that the homogeneity of the polymerized chromatographic monoliths on all size scales from 1 mL to 40 L column. This is unprecedented socio-economically very important due to the recognition of robustness and and reliability of monolithic technology. This facilitates decisions on the use of this technology for the biopharmaceutical industry.
F.04 Increase of the technological level
COBISS.SI-ID: 4003323