Human myoblasts robustly secrete IL-6 in basal and stimulated conditions. It is known that IL-6 has stimulatory effect on myoblast proliferation, suggesting its autocrine and paracrine action in muscle regeneration process. We found that addition of commercially available synthetic IL-6 had no significant effect on the in vitro proliferation neither in human myoblasts nor in the C2C12 muscle cell line in tested concetration range. In contrast, myoblasts treated with endogenous IL-6 present in conditioned medium of myoblasts that were previously stimulated with endotoxin lipopolisaccharide (LPS), have shown increased proliferation rate. This finding indicates that IL-6-mediated stimulation of myoblasts is complex and is probably more prominent in the presence of some factors that are secreted as response to LPS.
B.06 Other
COBISS.SI-ID: 28927705