We analysed the expression of genes, connected to response to infection with real-time PCR. We analysed expression of genes related to photosynthesis, carbohydrates metabolism and defence response. We also measured the amount of the virus in inoculated and noninoculated leaves. The results showed, that the plants respond differently to the infection and that we can expect a time shift in the response on the level of gene expression. We also showed the salicylic acid plays a crucial role in plant response to infection and that the depletion in the salicylic acid leads to the development of severe disease.
COBISS.SI-ID: 2492751
Fungi can be found in all aerobic environments, where they can colonise various surfaces or habitats performing different functions, which are not completely understood. Lot of fungi are generalists, meaning they can be found in all habits, while other fungi are specialists present in restricted habitats. Unusual habitats for fungi are habitats with extreme conditions, which are expected to prevent development of mycobiota. Nevertheless, in extreme habitats, e.g. Antarctic dry valleys, high Arctic glaciers, salt flats and salterns, hypersaline microbial mats and plant trichomes, very reach mycobiota was found and described. In plant trichomes we found several new, not previously described, fungi groups. With the use of new generation sequencing methods we can reveal the composition of the mycobiota in various habitats, however the role of the fungi in the extreme habitats remains unclear.
COBISS.SI-ID: 2462031
Method for functional analysis of fungal transcriptome was developed and used. Method allows identification of the role of the final gene product in fungal metabolism. Using this method, genes having a key role in fungal survival in extreme environments can be identified. Method was used for analysis of halotolerance of fungi Rhodotorula mucilaginosa and for detailed analysis of several genes, improving halotolerance of non-tolerant yeast Saccharomyces cerevisiae.
COBISS.SI-ID: 2514255
Potato virus Y presents the second most important microorganism, limiting potato production worldwide, since it causes drastic decreases in potato quality and quantity. Understanding of the physiological changes in potato infected with the PVY will contribute to easier development and breeding of resistant potato cultivars and to general understanding of the plant – pathogen interaction. We studied all available literature describing potato – PVY interaction. The gained knowledge and conclusions were compiled in a chapter published in Progress in Botany (Springer).
COBISS.SI-ID: 2662735