A viroid disease syndrome that was recently described on Slovenian cultivar ‘Celeia’ was investigated. The disease was transferred using biolistic approach to Czech hop under isolated experimental conditions. A complex of strong symptoms appeared on cultivar ‘Osvald’s 72’ that was quite comparable to cultivar ‘Celeia’; low plant fitness strong stunting, leaf malformation, small (tiny) leaves and frequently visible leaf mosaic, which was not associated with virus infection. Hop stunt viroid (HpSVd) was analyzed in detail in the inoculated Czech hop that survived initial infection. It was confirmed that a population of viroid was detectable in the leaves with a clearly dominant HpSVd-cucumber pale fruit viroid-like (HpSVd-cuc) sequence. Besides HpSVd, also traces of truncated Citrus viroid (CVd IV) were detected in surviving biolistically infected hop samples. In some samples, HpSVd was detectable by Northern blots, but not by RT PCR, suggesting some viroid sequence change(s) upon adaptation in hop. An expression of several pathogenesis-associated marker genes were investigated. It was found that experimentally induced viroid syndrome in ‘Osvald’s 72’ led to gene deregulation and especially to strong depression of HlbHLH2 transcription factor participating in metabolome regulation in hop.
COBISS.SI-ID: 632716
Results were actively presented at several conferences: RADIŠEK, Sebastjan, OSET, Matej, ČERENAK, Andreja, JAKŠE, Jernej, KNAPIČ, Vlasta, MATOUŠEK, Jaroslav, JAVORNIK, Branka. Research activities focused on hop viroid diseases in Slovenia. V: SEIGNER, Elisabeth (ur.). Proceedings of the Scientific Commission [of the] CICH - IHB - IHGC, International Hop Growers` Convention, Kiev, Ukraine, 4-9 June 2013, (Proceedings of the Scientific Commission, ISSN 1814-2192, 2013). Wolnzach: Scientific Comission, I.H.G.C., 2013, str. 58. [COBISS.SI-ID 7617913] POKORN, Tine, RADIŠEK, Sebastjan, JAVORNIK, Branka, JAKŠE, Jernej. Using small RNA technology to identify viroids on hop. V: SEIGNER, Elisabeth (ur.). Proceedings of the Scientific Commission [of the] CICH - IHB - IHGC, International Hop Growers` Convention, Kiev, Ukraine, 4-9 June 2013, (Proceedings of the Scientific Commission, ISSN 1814-2192, 2013). Wolnzach: Scientific Comission, I.H.G.C., 2013, str. 59. [COBISS.SI-ID 7618169] MATOUŠEK, Jaroslav, PATZAK, J., DURAISAMY, S. G., KOCÁBEK, T., KROFTA, Karel, PIERNIKARCZYK, R.J.J., RADIŠEK, Sebastjan, JAKŠE, Jernej, STEGER, G. Regulation of lupulin biosynthesis by hop transcription regulation factors and some strategies to enter the regulation network. V: SEIGNER, Elisabeth (ur.). Proceedings of the Scientific Commission [of the] CICH - IHB - IHGC, International Hop Growers` Convention, Kiev, Ukraine, 4-9 June 2013, (Proceedings of the Scientific Commission, ISSN 1814-2192, 2013). Wolnzach: Scientific Comission, I.H.G.C., 2013, str. 74-77. [COBISS.SI-ID 7618425] RADIŠEK, Sebastjan, PERSOLJA, Jolanda, JAKŠE, Jernej, JAVORNIK, Branka, MATOUŠEK, Jaroslav, PAVLIČ, Ema, KNAPIČ, Vlasta. Pojav viroidne zakrnelosti hmelja v Sloveniji = Occurence of hop stunt disease in Slovenia. V: TRDAN, Stanislav (ur.). Izvlečki referatov = Abstract volume. Ljubljana: Društvo za varstvo rastlin Slovenije: = Plant Protection Society of Slovenia, 2013, str. 48. [COBISS.SI-ID 7476089] JAKŠE, Jernej, POKORN, Tine, RADIŠEK, Sebastjan, JAVORNIK, Branka. Novel viroid in hops identified by massive-scale rna sequencing. V: International conference [of the] Plant diseases and resistance mechanisms, Vienna, Austria, 20-22 February 2013 : programme and abstracts. Vienna: VIPCA - Vienna International Plant Conference Association, [2013], str. 51. [COBISS.SI-ID 7470713]
COBISS.SI-ID: 7618425