In doctoral thesis the regulation of cysteine cathepsins by cystatin F is presented, the cysteine protease inhibitor which only enters endosomal/lysosomal vesicles inactivating directly cysteine cathepsins. The work presents the mechanism of action of the inhibitor, its localisation in various immune cells, such as monocytes and macrophages, DCs, and NK cells, as well as its potential role in response to infection with H. pylori, in regulation of antigen presentation in DCs and in activation of NK cells during antitumour immune response.
D.09 Tutoring for postgraduate students
COBISS.SI-ID: 261103360Cathepsin H is a cysteine protease acting as amino- and endopeptidase. The aim of doctoral thesis was to determine the mechanism of its processing and to find its natural substrates, unknown till now. We showed that the activation of cathepsin H is an autocatalitic process starting with processing of pro-form to intermediate form and finally to mature form. Intermediate and mature forms exhibit aminopeptidase activity. This process is disturbed in some cells resulting in accumulation of the intermediate form. As a natural substrate of aminopeptidase activity the N-terminal end of talin was determined, a protein crucial for regulation of migration of immune cells through the activation of integrin receptors.
D.09 Tutoring for postgraduate students
COBISS.SI-ID: 262426880International patent application protect the application of native and recombinant macrocypins in plant protection and other potential applications such as regulation of immune response.
F.06 Development of a new product
COBISS.SI-ID: 2741839