A novel orthoreovirus (MRV) was isolated from stool sample of a child, hospitalized for acute gastreonteritis, sharing the highest similarity to recently described strains in Italian and German bats. A successful approach was described, combining classical methods in virology and modern molecular method (next generation sequencing, NGS), for effective and timely diagnostics of viral infections. In addition, novel approach was presented for sample pre-treatment, including concentrating and purifying of viruses with a chromatographic method, enabling improved target nucleic acid recovery in NGS application.
COBISS.SI-ID: 2886735
In this article rotavirus strain G6P[11] was described with full genome analysis. Rotavirus strain was found in a stool sample of a child with gastroenteritis. G-P genotype combination G6P[11] determined in the detected strain was already described as the most prevalent genotype found in bovine rotaviruses. The whole genome analysis revealed high identity in all genome segments to bovine rotavirus strains, thus confirming the direct interspecies transmission from bovines to human. Whole genome phylogenetic analysis did not confirm genome reassortment between human and animal rotaviruses.
COBISS.SI-ID: 30182617
The objective of our study was to assess the significance of HCoVs in the etiology of acute gastroenteritis (AGE) in children (6 years of age. Stool samples and nasopharyngeal (NP) swabs collected from 260 children hospitalized for AGE and 157 otherwise healthy control children were tested for the presence of four HCoVs using molecular methods. HCoVs were more frequent in patients with AGE than in controls (23/260, 8.8% versus 4/151, 2.6%; odds ratio, OR 3.3; 95% confidence interval, CI 1.3–10.0; P = 0.01). Although HCoVs were more frequently detected in patients with AGE than in the control group, high prevalence of HCoVs in NP swabs compounded by their low occurrence in stool samples and detection of other viruses in stool samples, indicate that HCoVs probably play only a minor role in causing gastrointestinal illness in children (6 years old.
COBISS.SI-ID: 30419417