Protein structures evolved through a complex interplay of cooperative interactions, and it is still very challenging to design new protein folds de novo. Here we present a strategy to design self-assembling polypeptide nanostructured polyhedra based on modularization using orthogonal dimerizing segments. We designed and experimentally demonstrated the formation of the tetrahedron that self-assembles from a single polypeptide chain comprising 12 concatenated coiled coil–forming segments separated by flexible peptide hinges. This design platform provides a foundation for constructing new topological polypeptide folds based on the set of orthogonal interacting polypeptide segments.
B.04 Guest lecture
COBISS.SI-ID: 5340698As lecture we presented project achievements on the field of mechanism of TLR5 activation with flagellin.
B.04 Guest lecture
COBISS.SI-ID: 36980229We introduced the problems related to inovations within Slovenia region.
F.35 Other
COBISS.SI-ID: 5307162