We found that after LPS stimulation MARCKS moves from the cell membrane to the endosomes where it co9localizes with LPS. In this way, MARCKS can neutralize the ability of TLR4 activation by LPS, TLR4 since it is located in the same vesicles as MARCKS, which is a cytosolic protein.
COBISS.SI-ID: 4930586
We compared the ability of CLIP and iCLIP methods to identify UV-induced RNA sites interacting with proteins. The comparison was done on six published and two new experimental data sets. Over 80% of iCLIP reads truncated at the cross-link site, while only 10% of CLIP reads included a deletion, which is an indication for cross-linking. We have demonstrated that iCLIP provides a higher positional precition and is able to identify more RNA sites interacting with the given protein.
COBISS.SI-ID: 9319764