Method for enhanced production of recombinant proteins in plants or plant cells solves a problem of complex protein production with fast, reliable and economically viable solution. Preferably in N. tabacum plants it is introduced at least one polynucleotide comprising a sequence coding a protein to be produced, and at least one polynucleotide comprising a sequence for at least one further modulating protein or polyribonucleotide which increases the cell-cell permeability of the target cells, preferably from group of glycosidases, or it is introduced at least one polynucleotide which comprises the sequence for protein of interest and a sequence increasing the cell-cell permeability of the target cells, preferably from group of glycosidases. Polynucleotide sequences preferably originate from plant viruses, preferably TMV and/or PVX.
F.33 Slovenian patent
COBISS.SI-ID: 2483279The lecture was conducted during the workshop "Training Course on Molecular Diagnostics for Risk Assessment and Management of Genetically modified crops", which was held in New Delhi organized by the National Bureau of Plant Genetic Resources, Indian Council of Agricultural Research. The theme of the lecture was a presentation of basic research on potato-virus interaction and how this research led to applicative results.
B.04 Guest lecture
COBISS.SI-ID: 2489935The role of β-1,3-glucanase class III (Glu-III) gene was evaluated in the interaction between potato and potato virus YNTN using the functional genomics approach. We prepared transgenic potatoes overexpressing Glu-III. After PVYNTN inoculation differences in viral spread were observed between transgenic lines over-expressing Glu-III and non-transgenic lines. Differences in the speed of viral spread were correlating with differences observed in the expression of selected marker genes. We also developed a new method ) for detection of genetically modified organisms (GMO), named NAIMA (NASBA Implemented Microarray Analysis.
D.09 Tutoring for postgraduate students
COBISS.SI-ID: 261457920We evaluated role of mitogen-activated protein kinases (MAPKs) in response of non-transgenic potato cv. Rywal (hypersensitive resistance) and transgenic NahG-Rywal (salicylic acid deficient) to Potato virus Y (PVY) infection, using functional genomics approaches. MAPK signalling is one of the key regulators of plant response to pathogen attack. It is triggered in a form of a cascade, where three kinases are sequentially phosphorylated, which results in activation of defence genes and proteins. Negative regulators of the cascade are MAPK phosphatases. We analysed phylogenetic relationship of potato MAPKs in comparison to the model plant species A. thaliana and observed poorer representation of MAPKs in potato. The expression results of the non-transgenic potato cv. Rywal and transgenic NahG-Rywal in defence response against PVY show three different expression patterns. Most commonly, the genes are repressed in Rywal and induced in NahG-Rywal. We selected three differentially expressed genes for further functional analysis: MAP kinase kinase 6 (MKK6), MAP kinase (wound-induced protein kinase, WIPK) and MAP kinase phosphatase 1 (MKP1). Protein StMKK6, under the control of its native promoter, accumulated in nucleus after the infection. Using bimolecular fluorescent complementation (BiFC), we did not find any substrates of StMKK6 protein. Additionally, silencing of StWIPK in the transiently transformed plants of S. venturii did not have any effect on the spread of the GFP-marked PVY.
D.09 Tutoring for postgraduate students
COBISS.SI-ID: 818039In the show we have presented the transient transformations of plants and how we change the gene expression in plants. We have shown an example of localization studies and the possibility of plant protein production (e.g. for vaccine production).
B.06 Other
COBISS.SI-ID: 2771279