The aim of the thesis was to prepare PVA nanofibers with incorporated antimicrobial active ingredient levofloxacin, and thus develop a modern wound dressing that would improve healing by two synergistic approaches. Besides nanofiber preparation by electrospinning, a particular attention in the experimental work was given to the investigation of physical methods for stabilization of nanofibers in aqueous environment, and to the setting of models for monitoring the nanofibers’ hydration and erosion as well as release of the drug. In the end, the impact of prepared nanocomposite system on the growth of keratinocytes was evaluated. Our results showed that the addition of levofloxacin increases the viscosity and conductivity of PVA solutions, which consequently leads to the formation of thinner nanofibers. It was proved that each and every selected method of physical stabilization (soaking in nonsolvents, methanol and ethanol respectively, and thermal stabilization) ensure the preservation of fibrous structure in aqueous medium for at least 5 days, but the latter considerably affect the complete delivery system. Results namely indicate that the type of stabilization can lead to the change in diameter and porosity of nanofibers as well as significantly increase the crystallization of the incorporated drug, stimulate its movement from the interior to the surface, and determines its release profile. In the context of in vitro studies, we found out that keratinocytes grown on the nanofibrillar support develop altered morphology. Beside mentioned diploma thesis another three diploma thesis were finished in the scope of the project research in the year 2012 [COBISS.SI-ID 3235441],[COBISS.SI-ID 3219057], [COBISS.SI-ID 3294065]).
D.10 Educational activities
COBISS.SI-ID: 3218801The achievement represents one of the 7 scientific contributions, which were presented at 4 international conferences in the year 2012 (8th World Meeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology, Istanbul, Turkey; 9th Central European Symposium on Pharmaceutical Technology: nanopharmaceuticals and nanomedicine, Dubrovnik, Croatia; 9th International Conference and Workshop on Biological Barriers - in vitro and silico Tools for Drug Delivery and Nanosafety Research, Saarbrücken, Germany and SLONANO 2012, Ljubljana, Slovenia). The objectives of the presented research were preparation of electrospun chitosan based nanofibers from physicochemical characterized polymer solutions and in vitro evaluation of their biocompatibility as a prerequisite for their application in wound therapy. The research revealed that the relative humidity during electrospinning should be strictly controlled to achieve beadless nanofibers. The composition of polymer blend determines nanofiber stability in aqueous environment, and the viscosity of solution affects the thickness of produced nanofibers. Chitosan based nanofibers supported cell growth in vitro, therefore, showing a good potential for in vivo application in management of wound healing.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 3224177Educational TV program shows the safety issues and hazards of nanoparticles and also other nanomaterials, including nanofibers. The project leader prof. dr. Juliana Kristl together with other scientists involved in nanoparticle research clearly and understandably introduce the subject to the general public.
F.35 Other
COBISS.SI-ID: 2569039