A LA-ICP-MS method based on a 213 nm Nd:YAG laser and a quadrupole ICP-MS was developed for mapping of mercury in root cross-sections of maize (Zea mays L.) to investigate the mechanism of mercury uptake from soil and its potential translocation to the edible parts. Conventional rastering was found to be unusable due to sorption of mercury onto the internal parts of the LA device, giving rising to memory effects resulting in serious loss of resolution and inaccurate quantification. Spot analysis on a virtual grid on the surface of the root sections using washout times of 10 s in between spots greatly alleviated problems related to these memory effects.
COBISS.SI-ID: 2821455
LA-ICP-MS performance data are reported for a custom-made low-dispersion laser ablation cell. A 99% washout of the aerosol was achieved in ca. 6 ms, enabling separated pulse responses at frequencies up to 200–300 Hz. In addition, the cell employed supports a post-acquisition methodology for the deconvolution of overlapping ablation positions in scanning mode resulting in a lateral resolution in the order of 0.3 +/- 0.1 micrometer.
COBISS.SI-ID: 5631258
We studied the impact of 10 uM Cd or 50 uM Zn treatments on ion distribution in leaves of a metallicolous population of the non-hyperaccumulating species Zygophyllum fabago at tissue and cell level, and the consequences on the plant response through a combined physiological, proteomic and metabolite approach. Microproton-induced X-ray emission and laser ablation inductively coupled mass spectrometry analyses indicated hot spots of Cd concentrations in the vicinity of vascular bundles in response to Cd treatment, essentially bound to S-containing compounds as revealed by extended X-ray absorption fine structure and non-protein thiol compounds analyses. A preferential accumulation of Zn occurred in vascular bundle and spongy mesophyll in response to Zn treatment, and was mainly bound to O/N-ligands.
COBISS.SI-ID: 2984527
The distribution of Zn in vegetative and reproductive organs of adult Kosteletzkya virginica plants, and the NaCl influence on this distribution were studied. Plants were cultivated in a nutrient film technique system, from seedling stage until seed maturation in a control, Zn (100 uM), NaCl (50 mM) or Zn + NaCl medium. Zinc distribution in reproductive organs was estimated by laser ablation inductively coupled plasma-mass spectrometry procedure. Adult plants accumulated up to 2 mg/g DW Zn in the shoots. Zinc was present in seed testa, endosperm and, to a lower extent, in embryo. Additional NaCl induced a chalazal retention of Zn during seed maturation and reduced final Zn seed content.
COBISS.SI-ID: 2812495
A multi-elemental 3D laser ablation-ICP-mass spectrometry mapping procedure for high resolution depth information retrieval to investigate surface layer phenomena is presented. The procedure is based on laser drilling on a virtual grid on the surface, followed by extraction of depth maps along the z-axis (for each element monitored). Using a burst of 50 laser pulses at 1 Hz on each point of the grid, a penetration rate of ca. 150 nm per pulse (in glass) was obtained and a lateral resolution in the order of the laser beam diameter.
COBISS.SI-ID: 5202714