To gain insight into the responses of sensitive bacterial populations to colicin M in the natural environment and when applied as an antimirobial agent we studied the response of an E. coli strain to ColM subinhibitory concentrations on a global gene expession scale using DNA microarrays and physiological tests. Transcriptomic analysis showed increased expression of envelope, osmotic and other stress responses, including the two-component system CreBC genes, genes for extracellular polysaccharides and decreased expression of genes for motility. In addition, expression of the colicin K, cka gene was studied and found that protein IscR stabilizes protein LexA at the promoter and by cooperative binding of two LexA dimers induces a lag in transcription following induction of the SOS response.
D.09 Tutoring for postgraduate students
COBISS.SI-ID: 773239Isolates assigned to the phylogenetic group B2 encoded a higher number of virulence factors, including usp than isolates of other groups. colicins E6, E7, K and M efficiently inhibited growth of uroseptic E. coli isolates. the most efficient was colicin E7 as it inhibited growth of 87% of the tested isolates. Employing an appropriate combination of colicins it was possible to inhibit growth of 98% of the investigated strains.
D.09 Tutoring for postgraduate students
COBISS.SI-ID: 739959Strain ŽP is a modified probiotic strain Nissle coding for synthesis of the E7 bacteriocin on a conjugative plasmid and immunity against the E7 bacteriocin on the chromosome. The colicin destroys pathogenic E. coli strains following conjugal transfer of the plasmid. The described strain bypasses resistance against the antimicrobial agent.
F.33 Slovenian patent
COBISS.SI-ID: 2853455In the year 2011 in Germany and several other European countries 40 people died due to an outbreak of enterochemorrhagic E. coli. The strain provoking the outbreak was idnetified as enteroaggregative E. coli that had aquired via horizontal gene transfer DNA for the production of the Shiga-like toxin.
F.35 Other
COBISS.SI-ID: 258514176Textbook for microbial pathogenesis
D.10 Educational activities
COBISS.SI-ID: 2965583