We report a new method for the synthesis of biarsenical reagents, which are then used to monitor murine PrP (mPrP) misfolding. We introduced tetracysteine (TC) tags which bind biarsenical compounds into mPrP and devised a quantitative protease-free method for following PrP conversion, based on the ability of the biarsenical reagent to differentiate between the monomeric and fibrilized form of TC-tagged PrP, and showed that TC-tagged mPrP could be detected on transfected cells, thereby expanding the potential use of this method for the detection and study of conformational diseases.
COBISS.SI-ID: 4377626