On invited lecture I presented the study of gene expression of lung tumours, preliminary results of gene expression analysis on DNA microarrays and possible causes for detected differences in expression. We also presented differences in expression profiles between KRAS mutated and KRAS non-mutated tumours, correlations between expression of COX-2 and hTERT and gender of the patients and different molecular changes in tumours associated with histological differences.
B.04 Guest lecture
COBISS.SI-ID: 26661337Analysis of gene expression revealed that LATS2, STK11 and S100A2 gene expression is significantly different in different lung tumour types. With analysis of P53 status we were able to place novel alterations in STK11 and S100A2 as early events in lung carcinogenesis and alterations in LATS2 as late. In all three genes we identified novel mutations and polymorphisms that are specific for type of lung tumours, proving existences of cancer type specific changes in those genes.
E.01 National awards
COBISS.SI-ID: 26045145In an attempt to find novel molecular differences between different types of tumours, we created a DNA microarray platform (MA) by which expression levels of 3060 genes involved in carcinogenesis and apoptosis are monitored. By means of MA, more than 40 genes, most of them involved in transcription or transcription regulation, were detected as having differential expression in comparison to adjacent healthy tissue. Our findings confirmed LATS2, STK11 and VIPR1 as tumour suppressors and identify novel changes and genes that clearly show differences between histotypes on a molecular level.
E.02 International awards
COBISS.SI-ID: 27113945