We show that self cleavage of LexA repressor is prevented by binding to specific DNA operator targets, depends on LexA dissociation from the targets and, hence, this controls the SOS response. Distance measurements using EPR spectroscopy reveal that in unbound LexA the DNA binding domains sample different conformations, one of which is captured when bound to operator targets, precluding RecA interaction. Modulation of LexA activity changes the occurrence of persister cells in bacterial populations.
COBISS.SI-ID: 2368847
The chapter named The LexA regulatory system for the second edition of "Encyclopedia of Biological Chemistry" published by Elsevier. An invitation from Nancy L. Craig (Howard Hughes Medical Institute).