We have shown, by using confocal microscopy-based analysis and in vitro functional cellular tests that the amount of fused late endoytic compartments correlates with the potency of in vitro anti-tumor CTL responses, generated with electrofused tumor cell-DC hybridomas. This represents an important tool for predicting the antigen-specific stimulatory capacities of immunohybridomas that can be clinically used as anti-cancer vaccines.
COBISS.SI-ID: 25624281
Resveratrol, a polyphenol with pronounced antioxidative activity induces tolerogenic properties in DCs, especially when added during their in vitro differentiation from human monocytes. It decreases the expression of costimulatory and HLA class II molecules and increases the amount of ILT3 and ILT4 inhibitory molecules on the surface of the treated DCs. Additionally resveratrol decreases production of IL-12p70 and increases the secretion of IL-10. Treated DCs are poor activators of allogeneic T lymphocytes and they induce the onset of Il10-producing T cells.
COBISS.SI-ID: 2729841