The distribution of Potato virus Y (PVY) in the systemically infected potato (Solanum tuberosum) plants of the highly susceptible cultivar Igor was investigated. Virus presence and accumulation was analyzed in different plant organs and tissues using real-time polymerase chain reaction and transmission electron microscopy (TEM) negative staining methods. To get a complete insight into the location of viral RNA within the tissue, in situ hybridization was developed and optimized for the detection of PVY RNA at the cellular level.
COBISS.SI-ID: 2401359
Monoliths are chromatographic stationary phases, which were specially designed for efficient purification of large biomolecules, like proteins, viruses and DNA. Within the project a QA monolith in disk format has been used to purfy PVY virus from plant tissue. As alternative appliccation of monoliths, small scale monolithic butyl (C4) and styrene-divinyl benzene (SDVB) columns can be applied for reversed phase analyses of various degraded influenza viruses.
COBISS.SI-ID: 35012101