A lecture presented our finding that targeted inhibition of a fungal cytochrome P450, CYP53A15, yields novel treatments for fungal infections. This is an alternative approach, different from conventional inhibition of 14 alfa-demethylase (CYP51). We found that some naturally occurring antifungal phenolic compounds inhibit CYP53A15 of the pathogen Cochliobolus lunatus and a synergistic antifungal activity was observed in the presence of benzoate, a widely used agent for fungal growth retardation.
B.06 Other
COBISS.SI-ID: 4321562Two novel cytochrome P450 reductases, CPR1 and CPR2, were found in the filamentous fungus Cochliobolus lunatus. A phylogenetic analysis of these proteins was performed. High numbers of cytochromes P450, multiple CPRs and P450-fusion proteins reflect the complex biochemistry necessitated by filamentous growth. Functional characterization showed that CPRs are specific with only partly interchangeable physiological roles. This fact should be considered in studies of P450 reconstitution systems in research of virulence factors in pathogenesis, biotechnology and environmental bioremediation.
D.09 Tutoring for postgraduate students
COBISS.SI-ID: 246737408