This patent application describes the methods of biosynthetic engineering directed to produce novel medicinally important compounds of polyketide origin based on the chemobiosynthetic/mutasynthetic approach, where analogues of unnatural extender units are added into cultivation media of actinomycete cultures. These unnatural units are incorporated by polyketide synthases into polyketide backbones of compounds, such as the antibiotic erythromycin, thereby incorporating novel functional groups, which cannot be introduced by usual procedures of chemical semi-synthesis. In addition, these functional groups are chemically amenable and allow for subsequent derivatization of the obtained bioactive compounds. An important part of the patent application relates to production of novel erythromycin analogues and is based on the bioprocess, gene tools and transformation procedures, developed in Acies Bio for Saccharopolyspora erythraea as well as on new insights on this high-producing strain obtained by proteomic analyses.
F.09 Development of a new technological process or technology
COBISS.SI-ID: 4162936In the lecture "Biosynthetic engineering: new approaches to developing antimicrobial substances"new approaches of biosynthetic engineering of the enzymes involved in polyketide biosynthesis werte presented. Additionally new approaches for preparation of novel polyketide compounds, which possess a wealth of pharmacological effects, including antibacterial, antifungal, antiparasitic, anticancer and immunosuppressive activities, were presented.
B.04 Guest lecture
COBISS.SI-ID: 3577976In the scope of this scientific symposium different contributions of Slovenian and international research groups and companies were presented, with the focus on biosynthetic and metabolic engineering of diverse industrially important compounds as well as bioprocesses for their production. Several important achievements in production of different biological compounds with high importance for pharmaceutical and food industry were described. In addition, mechanisms of action, regulation of biosynthesis and strain improvement using metabolic engineering were presented for different compounds including erythromycin.
B.01 Organiser of a scientific meeting
COBISS.SI-ID: 258027776