The effect of temperature on signal molecules production in Vibrio sp. was studied in diploma thesis. Signal molecules isolated from the spent media of the wild type strain Vibrio sp. were extracted with acidified ethyl acetate. AHL molecules present in spent media were determined with TLC and biosensor strain E.coli JM109 (pSB401). For detection of AI-2 molecules a biosensor strain Vibrio harveyi BB170 was used. The results indicate that Vibrio sp. produces one type of AHL molecules, 3-oxo-hexanoyl-L-homoserine lactone (OHHL). It also produces AI-2 signal molecule. Production of AHL and AI-2 molecules is temperature sensitive.
D.11 Other
COBISS.SI-ID: 3946872The method for monitoring QS molecules during fermentation, which was developed in the project, was presented with an invited lecture at the international meeting of experts on yeast bioflavour production (2nd European Yeast Flavour Workshop, Cost Action FA0907 BIOFLAVOUR). The main contribution of the presented methodology is miniaturisation, efficacy and rapidness of the detection of yeast aromatic alcohols. Moreover, despite the miniaturisation, the method has still high sensitivity and specificity. The fermentation is processed in 2 ml bioreactors and detection is performed with HPLC and phenyl column of new generation. There is no need for special isolation of analites (e.g. extraction) or purification, therefore the optimized process exceedingly shorten, simplify and last but not the least reduce the cost of analysis (for approx. 100-1000 fold when compared with other methods in the field).
B.04 Guest lecture
COBISS.SI-ID: 3920248