The uptake of Se(IV) by Myriophyllum spicatum, Ceratophyllum demersum and Potamogeton perfoliatus, and the effects of Se(IV) on their physiological and biochemical characteristics were studied. Plants were cultivated outdoors under semi-controlled conditions in water solution containing Na selenite (20 µg Se L−1 and 10 mg Se L−1). The higher concentration of Se lowered the photochemical efficiency of PSII in all species studied, while the lower concentration had no effect on any species. The higher concentration of Se lowered respiratory potential in M. spicatum. The concentration of Se in plants cultured in 10 mg Se(IV) L−1 ranged from 436 to 839 µg Se g−1 DM in M. spicatum, 319 to 988 µg Se g−1 DM in C. demersum and 310 to 661 µg Se g−1 DM in P. perfoliatus. The amount of soluble Se compounds in enzyme extracts of high Se treatment was 27% in M. spicatum, 41% in C. demersum and 35% in P. perfoliatus. Se compounds were determined using HPLC–ICP-MS. It was observed that the applied Se(IV) was mainly transformed to insoluble Se.
COBISS.SI-ID: 2702671
For the study, cabbage (Brassica oleracea var. capitata L.) and red cabbage (Brassica oleracea var. capitata L. f. rubra) were treated with Na selenate. Cabbage was foliarly sprayed twice with 20 mg Se(VI) L–1, while red cabbage was fertilized twice with 0.5 mg Se(VI) L–1. Despite the high dose of Se, no toxic effects were observed on cabbage plants. The total Se concentration in cabbage leaves was 4.80 ± 0.25 μg Se g–1 (DM) and in red cabbage 0.96 ± 0.04 μg Se g–1 (DM). Soluble Se compounds were extracted from parts of cabbage with protease XIV, resulting in 49 % of soluble Se from roots, 59 % from leaves, and 65 % from stems. In red cabbage, the corresponding figures were 28 % of soluble Se in roots, 31 % in stems, and 43 % in leaves. Se species were determined in the enzymatic extracts using ion exchange high-performance liquid chromatography-inductively coupled plasma-mass spectrometry (HPLC-ICP-MS). The main Se species found in all parts of cabbage and red cabbage was selenomethionine (SeMet).
COBISS.SI-ID: 2492495
The ability of the widely distributed aquatic moss Fontinalis antipyretica to take up Se from water was studied. Nine locations in the Notranjska region (Slovenia) with different land use in the catchment were sampled for water and moss in the year 2010 in spring, summer and autumn. The concentrations of Se in water at all locations did not exceed 0.2 nanograms mL−1. F. antipyretica took up Se in the range between 345 and 2250 ng g−1. All results for Se are expressed on dry matter basis. The Se content varied depending on the location and season. The highest concentration (2250 plusminus170 ng g−1) of Se was found in the Žerovniščica stream that flows through an agricultural area with dairy farming. The fraction of insoluble Se compounds in the residue after enzymatic hydrolysis using protease (XIV) was around 75%. Soluble Se compounds in the enzymatic extract of F. antipyretica were separated and measured using HPLC coupled to ICP-MS. Se(IV) and Se(VI) were found but no organic Se compounds were detected, even at the highest concentration.
COBISS.SI-ID: 2649167
To investigate the impact of Se on Tartary buckwheat (Fagopyrum tataricum Gaertn.) plants, the plant foliage was sprayed with 10 mg Se(VI) L-1 at the beginning of flowering. The Se was effectively assimilated by the plants and taken into the seeds, where its concentration was more than double that in untreated plants. The seeds were collected and sown to obtain the progeny of these Se-treated plants. To assess the physiological characteristics of control plants and these Se-treated progeny plants, the estimated respiratory potential via electron transport system (ETS) activity and the photochemical efficiency of photosystem II were measured. Three weeks after germination, the Se-treated progeny plants showed higher ETS activity compared to the controls. Through weeks 4 and 5, this high ETS activity approximately halved, and the difference in ETS activity seen at 3 weeks was lost. On the other hand, at week 4, the potential photochemical efficiency was higher in the Se-treated progeny plants than the controls. In adult plants, the leaves dry mass was significantly greater in the Se-treated progeny plants than the controls. This study demonstrates an impact of Se in Tartary buckwheat on the progeny plants of Se sprayed plants, as shown previously in pea plants.
COBISS.SI-ID: 2702927
The transformation of selenium (Se) in buckwheat sprouts grown from seeds soaked in various Se solutions was investigated. The extraction procedure was optimised by (a) using optimal extraction media (water, phosphate buffer, 0.1, 0.2, 0.3 M HCl, the enzyme protease alone or in combination with cellulase, amylase or lipase), and by (b) optimising the ratio between sample and enzyme. Optimal extraction efficiencies were obtained by hydrolysis with 0.3 M HCl and protease. Selenate, Se-methionine and selenite (traces) were detected in all supernatants, regardless of soaking solution.
COBISS.SI-ID: 23639335