Magnetic nanoparticles are examined as delivery systems for different molecules. The aim of our study was to evaluate physicochemical properties, cytotoxicity, cellular uptake and trafficking pathways of the customsynthesized magnetic nanoparticles. We managed to prepare in isotonic solution stable magnetic nanoparticles with a diameter of 10 nm, which were not cytotoxic in a broad range of concentrations. They were readily internalized into different cells, however exposure to magnetic field increased their cellular uptake, predominantly into malignant cells.
COBISS.SI-ID: 967035
The review article is a comprehensive review of preclinical and clinical studies on IL12 electrogene therapy of cancer. The preclinical studies have demonstrated that IL12 gene elctrotransfer is an effective approach as local or systemic treatment. Translation of preclinical studies into clinical trials in human and veterinary oncology has started with encouraging results that would hopefully lead to further investigation of this therapy, also in combination with other cancer treatment modalities.
COBISS.SI-ID: 980859
In the review paper are described applications of magnetic nanoparticles in oncology. Special emphasis is on gene therapy, magnetofection, which is a promissing local, nonviral delivery system for naked plasmid DNA into the tumors.
COBISS.SI-ID: 1071739
We synthesized superparamagnetic iron oxide nanoparticles (SPIONs), coated and functionalized with a double layer of endosomolytic polymers, polyacrylic acid (PAA) and polyethylenimine (PEI). Onto SPIONs-PAA-PEI we bound plasmid DNA carrying reporter gene for green fluorescent protein (pDNAGFP) or therapeutic gene for interleukin-12 (pDNAIL-12). Biocompatible SPIONs are suitable vectors for gene delivery via magnetofection into cells and tumors. Magnetofection of cells and tumors with pDNAGFP or pDNAIL-12, bound to SPIONs-PAA-PEI, was superior in transfection efficiency to commercially available SPIONs or lipofection, and comparable to electrically-mediated transfer. Antitumor effect was after magnetofection with pDNAIL-12, bound to SPIONs-PAA-PEI, comparable to that of electrically-mediated transfer. Magnetofection of tumors using SPIONs-PAA-PEI-pDNAIL-12 holds great potential for the further refinement aimed at cancer immuno-gene therapy since the safety and efficacy of correspondingly efficient electrically-mediated transfer is already being tested in clinical studies for gene therapy.
COBISS.SI-ID: 1237883