The conference presentation represents a part of results on the field of magnetofection - transfection of genes with magnetic nanoparticles- in cell cultures. The procedure is based on application of stable surface functionalized magnetic nanoparticles, which form complexes with plasmides of therapeutic and reporter genes, and with use of external magnetic system successfully transfer this plasmid into cell cytoplasm. Within this project activities on design and preparation of appropriate functionalized nanoparticles and magnetic systems were done, whereas further work was necessary for development of appropriate magnetofection protocols for use in in vitro and in vivo systems. Other contributions were presented as: Magnetofection of B16F1 cells with therapeutic gene for interleukin-12, Zavod za zdravstveno varstvo; Abstract book; 2011; Str. 216; Avtorji / Authors: Prosen Lara, Prijič Sara, Žnidaršič Andrej, Čemažar Maja, Serša Gregor (Cobiss ID 1157243) Magnetic nanoparticles-mediated in vitro gene delivery, SLONANO 2011 Book of abstracts; 2011; Str. 80; Avtorji / Authors: Prosen Lara, Prijič Sara, Žnidaršič Andrej, Čemažar Maja, Serša Gregor (Cobiss ID 1173627)
F.02 Acquisition of new scientific knowledge
COBISS.SI-ID: 1185403One of major current limitations of magnetic drug targeting is to achieve efficient concentration of magnetic carrier-drug complexes at the targeted sites due to poor mobility of nanoparticles in tissue structures, therefore it is essential to know particle mobility in a given enviroment. We addressed this problem with combination of experimental measurements and numerical modeling. We used gel magnetophoresis in order to measure motilities of different magnetic NPs (Co-ferrite, c-Fe2O3) in agarose gel. Numerical modeling using FEM method was used to determine appropriate settings of magnets, which generate sufficient magnetic field gradient. Further, we used the numerical modeling to evaluate the magnetic force on the NPs for different geometries. We obtained that one of crucial factors which determines final mobility in tissue is formation of larger aggregates of nanoparticles under physiological conditions and interaction of nanoparticles with surrounding matrix. These findings and experimental and numerical models will be applied for further study of biologically relevant systems.
F.02 Acquisition of new scientific knowledge
COBISS.SI-ID: 8747860