To the book Chemoprevention of cancer and DNA damage by dietary factors we contributed a chapter on Protective effects of beer and its constituents. In the prestigious journal The Lancet the book has been very well appreciated and has been warmly recommended to scientists in the field of nutrition and cancer pharmacology and to decision makers and opinion leaders in health authorities and the food industry, because it provides relevant, practical, and wellbalanced information on all matters relating to cancer prevention by dietary factors.
COBISS.SI-ID: 1963343
The contents of the HCAs are dependent on the presence of the skin and the method of grilling, with the formation HCAs seen to be extensive in the absence of the skin and with direct heat transfer. The choosing of an appropriate method of thermal treatment of chicken meat to minimize the formation of HCAs is one of the most important issues. Thus, the intake of HCAs from grilled chicken can be reduced by not consuming the skin only in the case of infrared grilling.
COBISS.SI-ID: 3635832
Xanthohumol (XN) is a hop derived prenylated flavonoid contained in beer that shows promising chemopreventive properties that have been so far studied in the in vitro experiments. In this in vivo study we showed that dietary supplemetation of rats by XN supress amino3methylimidazo[4,5f] quinoline (IQ)induced DNA damage in colonocytes and hepatocytes and formation of preneoplastic foci in livers and colons of rats. Since the effects were observed with low doses of XN which are reached after consumption of brews with high XN levels, our findings may be relevant for humans.
COBISS.SI-ID: 2240591
This article presents an approach to microarray data analysis using discretised expression values in combination with a methodology of closed itemset mining for class labeled data (RelSets). The approach was validated on two independent sets of twocolor microarray experiments using potato plants. Our results demonstrate that the two different analytical procedures, applied on the same data, are adequate for solving two different biological questions being asked. The used algorithms are freely available upon request to the authors.
COBISS.SI-ID: 2210383
In this study, we showed that XN was not mutagenic in Salmonella typhimurium TA98 and did not induce genomic instability in human hepatoma HepG2 cells. In the bacteria XN suppressed the formation of 2-amino-1-methyl-6-phenylimidazo[4, 5-b]pyridine (PhIP) and 2-amino-3,8 dimethylimidazo[4,5-f]quinoxaline (MeIQx) induced mutations in a dose dependent manner and in HepG2 cells it completely prevented PhIP and MeIQx induced DNA strand breaks at nanomolar concentrations. With the QRT-PCR gene expression analysis of the main enzymes involved in the biotransformation of HAAs in HepG2 cells we found that XN upregulates the expression of phase I (CYP1A1 and CYP1A2) and phase II (UGT1A1) enzymes. Further gene expression analysis in cells exposed to MeIQx and PhIP in combination with XN revealed that XN mediated up-regulation of UGT1A1 expression may be important mechanism of XN mediated protection against HAAs induced genotoxicity. Our findings confirm the evidence that XN displays strong chemopreventive effects against genotoxicity of HAAs, and provides additional mechanistic information to assess its potential chemopreventive efficiency in humans.
COBISS.SI-ID: 2470223