We published several new assays for detection of GMO in food and feed in a journal, which is listed in the category A''.
COBISS.SI-ID: 2989647
In a journal, which is listed in the category A'', we publishes a paper on macrocypins, cysteine protease inhibitors from the parasol mushroom Macrolepiota procera. Macrocypinswere evaluated for their adverse effects and their mode of action on the major potato pest Colorado potato beetle.They were shown to reduce larval growth when expressed in potato or when their recombinant analogues were added to the diet. Macrocypins target a specific set of digestive cysteine proteases, intestains. Additionally, protein-protein interaction analysis revealed potential targets among other digestive enzymes and proteins related to development and primary metabolism. No effect of dietary macrocypins on gene expression of known adaptation-related digestive enzymes was observed in CPB guts.
COBISS.SI-ID: 27291431
A first report of one-step reverse-transcription droplet digital PCR-based absolute quantification of a RNA virus (rotavirus) in different types of surface water samples. This new tool is fully amenable for the quantification of viruses in the particularly low concentrations usually found in water samples.
COBISS.SI-ID: 2990415
We report a molecular typing system of E. amylovora based on variable number of tandem repeats (VNTR) analysis (minisatellites). A Multiple-Locus VNTR Analysis (MLVA) was applied to E. amylovora isolates from bacterial collections representing global and regional distribution of the pathogen. Based on six repeats, MLVA allowed the distinction of 227 haplotypes among a collection of 833 isolates of worldwide origin. Three geographically separated groups were recognized among global isolates using Bayesian clustering methods. Analysis of regional outbreaks confirmed presence of diverse haplotypes but also high representation of certain haplotypes during outbreaks.
COBISS.SI-ID: 2982991
The ultrastructural colony architecture of six Clostridium difficile strains belonging to four different ribotypes (001, 027, 078/126 and 036) was studied by scanning electron microscopy (SEM).
COBISS.SI-ID: 20178184