Anticoagulant properties of annexin A5 are a consequence of crystallization, which forms the lattice over phospholipid surface. Crystallization of annexin A5 has been proven on homogeneous synthetic phospholipids. In our study, the crystallization of annexin A5 on inhomogeneous, naturally derived phospholipid surfaces has been reported for the first time. Crystallization of annexin A5 on different solid supported phospholipid bilayers was observed by atomic force microscopy. Our results support the putative role of annexin A5 crystal structures as possible antithrombotic shield.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 2526577Protein oxidation represents covalent modification of protein leading to the loss of physiologic functions of protein. Oxidation of autoantibodies is poorly investigated. For demonstration of oxidation, the amount of CO groups was measured. After electrooxidation in experimental conditions IgM and IgG fractions showed 5 and 2 times, respectively, higher amount of CO groups compared to nonoxydized controls. Modifications in aminoacid chains can influence the affinity of antibodies.
B.04 Guest lecture
COBISS.SI-ID: 9999999The immune response may change due to altered proteins or modifications of immunoglobulins. Oxidative processes may change immunoglobulins due to chemical alteration of the hypervariable region. Increased immunoreactivity as well as the avidity of IgG from healthy persons against ß2-glycoprotein I after being exposed to direct current was showed. Chemical stability of immunoglobulins and patient’s antioxidative status may influence the range of alterations induced by electrooxidation.
B.03 Paper at an international scientific conference
COBISS.SI-ID: 2526065The lecture included the description of antigens Ku p70/p80 on the surface of cellular membranes, the mechanisms of membrane asymmetry, cellular consequences when this system (including flippases, floppases and scramblases) is dysrupted and the pathological implications involved. Anti-Ku antibodies and their prevalence in autoimmune diseases was also addressed.
B.04 Guest lecture
COBISS.SI-ID: 26822873The lecture included the description and characterization of serum amyloid A, as a sensitive marker of inflammation, that can serve as an independent predictive marker of cardiovascular disease and even mortality following myocardial infarction. Antibodies against serum amyloid A have been identified and are proposed to serve as physiological regulators in homeostasis and could act as part of the natural antibody repertoire.
B.04 Guest lecture
COBISS.SI-ID: 26822617