Employing genome-wide gene expression approach in elucidating osteoporosis pathogenesis we found genes of antioxidative chain to be differentially expressed. Then we tested »oxygen stress hypothesis« by an in vitro assay on human osteosarcoma cell line cells treated with hydrogen peroxide. We found upregulation of the same genes involved in the response to oxidative stress. Our results present a novel list of genes and metabolic pathways that may be associated with the pathogenesis of osteoporosis
COBISS.SI-ID: 2678385
Oxidative damage is supposed to contribute to osteoporosis. We evaluated Slovenian elderly women and elderly men. All subjects were genotyped for the presence of GPX1 (antioxidant enzyme) gene polymorphisms. Haplotype analysis revealed that the 6-T haplotype is associated significantly with low BMD and with high plasma osteocalcin concentrations. This study shows for the first time that the polymorphisms of the GPX1 gene, individually and in combination, are associated with BMD and it implies the important contribution of the oxidative stress to pathogenesis of osteoporosis.
COBISS.SI-ID: 2864497
Adiponectin and leptin are associated with insulin resistance. In patients with diabetes type 2 (T2D) fasting plasma insulin, adiponectin and leptin were determined. Insulin resistance estimates were derived by the established equations and compared with the direct measurement of insulin resistance obtained with the euglycemic hyperinsulinemic clamp. The adiponectin-leptin ratio correlated best with the clamp derived sensitivity index compared to other surrogate measures of insulin resistance. The adiponectin-leptin ratio may become a new laboratory marker of insulin resistance in T2D.
COBISS.SI-ID: 26759385
Receptor activator of nuclear factor kappaB (RANK) is important in regulation of osteoclastogenesis. RANK gene polymorphisms were in determined 467 postmenopausal women and 117 elderly men. Significant associations of +35966insdelC with BMD at all sites were found in postmenopausal women, but not in men. Significant gene-gene interaction was proved for two RANK polymorphisms in combination with OPG and RANKL polymorphisms studied previously in postmenopausal women. Our results suggest that gene-gene interactions between RANK and OPG, and RANK and RANKL influence BMD in postmenopausal women.
COBISS.SI-ID: 2701425