The article deals with structural changes of Golgi apparatus during differentiation from basal to superficial cell layer in normal urothelium and in regenerating urothelium during regeneration induced by single cyclophosphamide treatment of mice. We have shown that reorganisation of Golgi is comparable in both models and it is differentiation dependent. With the higher stage of cell differentiation, Golgi becomes more and more complex, which is tightly correlated with the formation of urothelial plaques.
COBISS.SI-ID: 25923289
In this article, we have compared apical endocytotic pathway and activity of variously differentiated urothelial cells in culture. Quantitative analysis has shown that endocytotic activity is inversely related to the cell's differentiation stage. We have demonstrated that membrane-bound markers are delivered to lysosomes, while fluid-phase markers are seen only in early endocytotic compartments. We have provided the first evidence that in highly differentiated cells the uroplakin-positive membrane regions were excluded from internalization.
COBISS.SI-ID: 25479641
We have studied rat urothelium from postnatal days 0 to 14. Between postnatal days 7 and 10, intense apoptosis and massive desquamation has been detected, demonstrated by caspase activation and tight junctions' disruption, respectively. Cell loss is rapidly replaced by more mature urothelium.
COBISS.SI-ID: 25793241
Cyclophosphamide causes necrosis of urinary bladder urothelial cells, which is followed by regeneration, characterised by simple hyperplasia and appearance of partially differentiated superficial cells. We have investigated the effect of melatonin on urothelial regeneration. Melatonin prevented the development of hyperplastic urothelium, statistically significantly decreased proliferation and apoptosis and caused higher differentiation state of superficial urothelial cells.
COBISS.SI-ID: 25923545
In this review article, we have examined the traffic (exocytosis/endocytosis) of the apical plasma membrane during differentiation of urothelial cells. We have focused on the physiological and clinical significance of the apical plasma membrane traffic in bladder superficial urothelial cells.
COBISS.SI-ID: 25314521