Human epidermal growth factor receptor (EGFR) has emerged as an attractive target for cancer therapy. In this study, fluorescent, labeled magnetic, amino- or carboxyl-functionalized silica-coated maghemite nanoparticles were conjugated with epidermal growth factor (EGF) using different binding modes. The binding efficiency of EGF to the nanoparticles was measured by flow cytometry using specific anti-EGF antibody. The ability of EGF bioconjugates to target the EGF receptors was tested using EGFR over-expressing A431 cells in comparison to EGFR negative HeLa cells. Our results showed that bioconjugates where EGF was bonded by carbodiimide chemistry are the most effective for specific targeting of EGFR-expressing cells in vitro.
COBISS.SI-ID: 26704935
Magnetically-aligned BaFe12O19 films for self-biased applications in microwave devices can be prepared by electrically- or magnetically-directed assembly of BaFe12O19 nanoplates. The final magnetic alignment and the magnetic properties of the films, with thickness up to 30 m, depends on the interparticle interactions in suspensions and on the effect of an external field (magnetic or/and electric) on the particles. We showed that high quality films can be prepared by a strict control of the magnetic dipole interactions between the particles. This was achieved with the use of the particles of a particular size and with the control of the applied field.
COBISS.SI-ID: 26309671
The scientific paper the synthesis of dendrites of magnetic perovskite LaSrMnO3 (LSMO) is published for the first time. New mechanism of the dendrite nucleation was proposed. During hydrothermal treatment at lower temperatures hexagonal platelet crystals of a SrMnO3 - La solid solution with hexagonal perovskite structure form first. At higher temperature LSMO perovskite nucleates epitaxially at the edges of the hexagonal crystal and grows outwards forming dendrite structure. With further increase in the temperature causes further growth of the structure into dendrites of a hexagonal “snowflake” shape or the dendrites brake from the template and form structure with a specific shape of “pine trees”.
COBISS.SI-ID: 36823813
Members of the Research Program P2-0089 are being also involved into the nanotoxicology research as experts for nanoparticles. In the systematic study of influence of different carbon nanoparticles (carbon black, fularene, graphene oxide) we provided detailed characterization of the nanoparticles colloidal properties.
COBISS.SI-ID: 2848591
Ferromagnetic liquid crystals were predicted more than 40 years ago by Brochard in de Gennes. This work is the first experimental proof of their theory. Ferrimagnetic BaFe12O19 nanoplates are the basis of the ferromagnetic liquid crystal. Their synthesis was developed within the research program P2-0089. The crucial property of the incorporated magnetic nanoparticles is their plate-like shape that originates from their crystal structure, in addition to their size and magnetic properties. The synthesis of the applicable nanoparticles was developed under hydrothermal conditions in a combination with partial chemical substitution of Fe3+ with Sc3+. The final incorporation of the nanoparticles into a liquid crystal was made possible by the specific control of their surface properties. Due to the coupling between the liquid-crystal director and the magnetic moments of the nanoplates the optical properties of ferromagnetic liquid crystals can be tuned with electric or magnetic field.
COBISS.SI-ID: 27304231