The publication aims to explain the mechanism of GBM cytotoxicity of Trisenox (As2O3), otherwise an antileukemic drug. As2O3 triggers the autophagy via free radicals and simultaneously inhibits lysosomal Cathepsin B, causing apoptotic switch. We can drastically enhance this by inhibiting CatL, which we previously proved to have an antiapoptotic acitivity. This finding needs further confirmation in animal studies before it can be translated to clinics
COBISS.SI-ID: 2213967
In this contribution we described, along with the first author form the group of Rolf Bjerkvig, collaborating with many European laboratories, on the observation of MSC transformation. This has been previously described as spontaneous , but here we reported that this was not the case, but in fact the consequence of contamination with the tumour cells, kept in these laboratories. This is an important message warning against false results, when cells are not regularly tested by DNA fingerprinting to assure their identity.
COBISS.SI-ID: 2266703
In this work we studied how the most abundant stromal cells in glioblastoma (GBM), i.e. endothelial cells and macrophages influence the phenotype of GBM cells. Stromal cells did not impact proliferation of tumour cells, but enhanced their invasion and reduced the triggered apoptotic response. On the other hands, GBM cells stimulate proliferation of stromal cells. We concluded that stromal cells create then environment, which facilitates and contributes to the development of GBM. GBM cells stimulate the growth of these cells thereby assuring a better environment for tumour progression.
COBISS.SI-ID: 2086991
We have studied genotoxicity of two types of TiO2 nanoparticles: (25 nm: anatase, TiO2-An, and (100 nm rutile: TiO2-Ru. Human hepatoma HepG2 cells which was used as model. While neither TiO2-An nor TiO2-Ru affected the viability of HepG2 cells, both types induced intracellular reactive oxygen species (ROS) formation with TiO2-An being significantly stronger inducer of ROS than TiO2-Ru. DNA strand breaks and oxidative DNA damage were induced only by TiO2-An. TiO2 nanoparticles induced changes in the mRNA expression of DNA damage responsive genes,. TiO2 nanoparticles are therefore genotoxic.
COBISS.SI-ID: 24141607
Xantohumol (XN) is a hop derived prenylated flavonoid in beer. We monitored the impact of XN on the formation of amino-3-methyl-imidazo[4,5-f]quinoline (IQ) induced preneoplastic foci in liver and colon of rats. Supplementation of the drinking water with XN during carcinogen treatment led to a significant reduction of the number of GST-p+ foci and DNA damage in the liver and colon. Our findings indicate that XN protects against DNA damage cancer by the cooked food mutagen in rats what may be relevant also for humans.
COBISS.SI-ID: 2240591