We have contributed a chapter to the book Ecotoxicology Research Developments, where we have described the known biological activities of hepatotoxic and non-hepatotoxic cyclic cyanopeptides mainly from an ecotoxicological point of view.
COBISS.SI-ID: 2096207
The immunostaining of cytoskeletal elements by using epifluorescence and confocal microscopy has confirmed that the lysis induced by n-hCCP expands from single temperate virus infected cells or cell groups, the focal points, to their immediate environment. Our in vitro experiments demonstrate that lysogen focal point formation, which follows induction by endogenous cyanobacterial cyclic cyanopeptides, could constitute, also in the natural environment, the basis for an rapid and extensive cyanobacterial bloom collapse. This is the most dangerous moment for human and environmental health.
COBISS.SI-ID: 2070607
We showed synergistic effects of organophosphorus pesticides (OPs) on benzo(a)pyrene (BaP)-induced genotoxicity in the bacterial reverse mutation assay, and in micronucleus assay in human hepatoma HepG2 cells. Based on the obtained results we propose that co-genotoxicity results from OPs mediated modulation of BaP metabolism, favouring the induction of aldo-keto reductase (AKR1C) enzymes known to catalyse the formation of DNA reactive BaP o-quinones and the production of reactive oxygen species.
COBISS.SI-ID: 2104655
V tem delu je opisan podroben proteomski pristop s ciljem odkriti, na proteomski ravni, ključne razlike med dvema tipoma glioblastoma –GBM: invazivnim in angiogenim. Ker sta to karaktorstični GBM lastnosti, je izredno pomembno ugotoviti ustrezne biološke označevalce. Ti so bili analizirani na podganjem modelu GBM. Na iTRAQu temelječ kvantitativni proteomski pristop, povezan z bioinformatiko je razkril povečano metabolično aktivnost in celično sporazumevanje v angiogeni, v primerjavi z invazivno obliko glioblastoma.
COBISS.SI-ID: 2043471
We described the preparation of GBM spheroid model and how the established model could serve for studying the invasive subpopulations of tumour cells. We succeeded in separation of this invasive subpopulation from organotypic primary cultures of GBMs from patients and determined its proteolytic profile that pointing on importance of cathepsin B, which is presumably postranslationally actived in the invasive cell phenotype. In the continuation of this work this model will be used to study tumour stem cells invasion by synthetic inhibitors of cathepsins which are the subject of this project.
COBISS.SI-ID: 2042447