ANGTDP-43 is a predominantly nuclear RNA-binding protein that forms inclusion bodies in FTLD and ALS diseases. Using iCLIP we found that TDP-43 preferentially bound long clusters of UG-rich sequences in vivo. Analysis of RNA binding by TDP-43 in brains from subjects with FTLD revealed that the greatest increases in binding were to the MALAT1 and NEAT1 noncoding RNAs. Results of alternative splicing regulated by TDP-43 highlighten the importance of TDP-43 for the regulation of splicing in the brain.
COBISS.SI-ID: 8278100
We showed that the pore-forming protein perforin (PFN) is able to induce invaginations and formation of complete internal vesicles in giant and large unilamellar vesicles. Membrane capacitance measurements showed that PFN is able to increase a planar lipid membrane surface area in the absence of pore formation. Addition of PFN to Jurkat cells caused the formation of internal vesicles prior to pores and triggered an endocytosis-like event in addition to pore formation.
COBISS.SI-ID: 2493519
We showed an association of desmosomal cadherin desmocollin 2 (Dsc2) in cholesterol-enriched fractions that contain membrane raft markers caveolin-1 and flotillin-1 and the novel raft marker ostreolysin. The results of immunofluorescence microscopy confirmed colocalization of Dsc2 and ostreolysin. Partial depletion of cholesterol with methyl-beta-cyclodextrin disturbed desmosome assembly and significantly reduced the strength of cell-cell junctions and partially released Dsc2 from membrane rafts. Results indicated that a pool of Dsc2 is associated with membrane rafts labeled with ostreolysin. We showed thus that cholesterol could be a potential regulator of promotes desmosome assembly.
COBISS.SI-ID: 27616729