Volatile organic compounds (VOCs) play an important role in the chemistry of the atmosphere and in biogeochemistry. They contribute to the oxidative capacity of the atmosphere, particle and air pollutants, as well as to the production of greenhouse gasses (for instance ozone). Among analytical techniques for their determination in the atmosphere gas chromatography coupled with mass spectrometry (GC–MS) offers several advantages. However, for an accurate quantification calibration with standard substances is necessary. A quantitative structure–property relationship (QSPR) model for the prediction of MS response factors was developed on basis of our experimental measurements for the quantification of ozone precursors present in the atmosphere. A linear correlation between chemical structures and response factors was established by using a 7-parameter MLR model. The average error in the prediction of response factors was calculated by cross-validation procedure and was below 20%, which is sufficient for the determination of VOCs in the air. The proposed procedure is time consuming so it is more suited for the quantification of tentatively identified organic compounds during the reprocessing of MS chromatograms in cases when the original sample is no longer available.
COBISS.SI-ID: 1448796
The possible effects of volatile organic compounds (VOCs) on the durability of model and real historic papers was evaluated. Possible protective effects of the use of activated charcoal cloth, oxygen removal, and of various chemisorbents were also investigated. The results suggest that particularly VOCs with acidic or oxidisable functions can have a strong effect on degradation of cellulose. The removal of VOCs from the immediate environment can have a pronounced beneficial effect on papers emitting VOCs more intensively. The results have implications for storage of paper-based heritage in enclosures.
COBISS.SI-ID: 34838277
Monoliths are chromatographic stationary phases, which were specially designed for efficient purification of large biomolecules, like proteins, viruses and DNA. In this work, the small scale monolithic butyl (C4) and styrene-divinyl benzene (SDVB) columns were applied for reversed phase analyses of various degraded influenza viruses. The binding of the HA1 subunit of haemagglutinin to the monolithic columns was confirmed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and the Western blot. The working linear range was determined as 1.60×1010 viral particles/mL to at least 1.64×1011 viral particles/mL, the limit of detection was found to be 2.56×109 virus particles/mL and the limit of quantification was 5.12×109 virus particles/mL. The analytical HPLC method developed with the H1N1 virus was also applicable for the analytics of the HA1 subunit of H3N2 influenza virus and the influenza B virus.
COBISS.SI-ID: 35012101