An HPLC/MS/MS method was developed for identification of impurities in gentamicin. The HPLC was performed on a Synergy Hydro-RP column using 50mM trifluoroacetic acid (TFA), pH 2 adjusted with ammonium solution and methanol as mobile phase. Atmospheric pressure chemical ionization (APCI) was used and product mass spectra of protonated molecules were acquired. Seventeen impurities were detected in gentamicin.
D.09 Tutoring for postgraduate students
COBISS.SI-ID: 33588229Surface oxidation of the duplex stainless steel DSS alloy 2205 was studied by X-ray photoelectron spectroscopy (XPS) and SEM imaging. The experiments were performed on the alloy after controlled oxidation with oxygen atoms created in an inductively coupled plasma. Compositions of the modified oxidized surfaces were obtained from XPS survey scans, and the chemistries of selected elements from higher energy resolution scans of appropriate peaks. Different Fe/Cr/Mn oxidized layers and different oxide thicknesses were observed and correlated with temperature.
F.09 Development of a new technological process or technology
COBISS.SI-ID: 707498In a review article, an overview of methods for analysis and sample preparation published in the last ten years is given for the most often encountered mycotoxins in different samples. Special emphasis is on liquid chromatography with fluorescence and mass spectrometric detection, while in the field of sample preparation various solid-phase extraction approaches are discussed. Various issues are critically discussed: sampling and choice of representative sample, sample preparation and possible bias associated with it, specificity of the analytical method and critical evaluation of results.
F.02 Acquisition of new scientific knowledge
COBISS.SI-ID: 30096389Essential oils are in the pharmacopoeas defined as volatile constituents of plants separated by hydrodistillation (HD), steam distillation (SD) or pressing. Solvent extraction (SE) is used for more delicate essential oils. Modern established and emerging extraction techniques for essential oils aim to overcome these problems. Their advantages, disadvantages and applicability are discussed in this review, which covers mainly the scientific literature published in the last decade.
F.03 Increased qualifications of the research and development staff
COBISS.SI-ID: 33715461Modification of standard method for measurements of organochlorine pesticides (OCPs) in food products was developed. The modifications were made in the extraction step in which Soxhlet extraction was used. For the clean-up step smaller columns were introduced and for the preconcentration step a Kuderna-Danish technique was proposed. Introduced modifications improved the recovery values for all analyzed pesticides over 60% and the reproducibility was in the range of 10% (RSD). The method is suitable for the determination of OCPs in meat products with high content of fat.
F.02 Acquisition of new scientific knowledge
COBISS.SI-ID: 3736952