rHuEpo is used in medicine; it acts as a stimulus for production of red cells. Detection method that could differentiate between endogenous and exogenous Epo was developed in 2000, when the method called isoelectric focusing (IEF) was first used for detection of doping in sport. The problem of rHuEpo detection is structural similarity to endogenous Epo and low concentration of Epo in human blood and urine. IEF is currently not used as diagnostic method in clinical samples. It has potency to be used as method to identify the effects of rHuEpo and other therapy on endogenous Epo expression.
COBISS.SI-ID: 26200537
Endogenous Epo production in cancer patients may be altered due to chemotherapy, anaemia, rHuEpo treatment, TNF and IL-1. Urine and blood samples were obtained from two metastatic cancer patient treated with chemotherapy with or without rHuEpo, analysed with isoelectric focusing (IEF) with the aim to determine the effect of chemotherapy and rHuEpo treatment on the level of endogenous Epo.
COBISS.SI-ID: 26050265