The expression of bacterial polyketide synthase gene clusters is often controlled by a number of different families of regulatory proteins. In this article, we have undertaken a comparative bioinformatic analysis of the regulatory genes present in the oxytetracycline and chlortetracycline gene clusters of Streptomyces rimosus and S. aureofaciens. We have identified a new LAL(luxR)-family regulatory gene, otcG, in the otc gene cluster, that is not present in the ctc gene cluster. By gene disruption and over-expression experiments we have demonstrated a positive role of otcG in OTC biosynthesis.
COBISS.SI-ID: 3608696
Tetracycline antibiotics are industrially and clinically important antibiotics with broad spectrum of activity. Understanding of the regulation of tetracycline antibiotics production is very important for industrial production of these antibiotics. In this publication, results regarding a regulatory element with positive influence on the production of oxytetracycline were presented.
COBISS.SI-ID: 3580280